Detailed view for Tb927.2.3080

Basic information

TDR Targets ID: 55098
Trypanosoma brucei, cytochrome b5-dependent oleate desaturase
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.2757 | Length (AA): 408 | MW (Da): 47079 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 6

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00487   Fatty acid desaturase

Gene Ontology

Mouse over links to read term descriptions.
GO:0008732   L-allo-threonine aldolase activity  
GO:0016491   oxidoreductase activity  
GO:0006633   fatty acid biosynthetic process  
GO:0006629   lipid metabolic process  

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
13 76 3zlc (A) 74 156 31.00 0.82 0.04 0.296763 1.29
166 216 3n9y (A) 24 72 37.00 0.41 0 0.3259 1.24

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_129048)

Species Accession Gene Product
Arabidopsis thaliana AT3G12120   omega-6 fatty acid desaturase
Brugia malayi Bm1_38160   Fatty acid desaturase family protein
Candida albicans CaO19.7765   one of two likely delta-12 fatty acid desaturases similar to E.nudulans OdeA
Candida albicans CaO19.4933   one of two likely delta-12 fatty acid desaturases similar to E.nudulans OdeA
Candida albicans CaO19.12399   one of two likely delta-12 fatty acid desaturases similar to E.nudulans OdeA
Candida albicans CaO19.118   one of two likely delta-12 fatty acid desaturase genes similar to E.nudulans OdeA
Caenorhabditis elegans CELE_Y67H2A.8   Protein FAT-1
Caenorhabditis elegans CELE_W02A2.1   Protein FAT-2
Leishmania braziliensis LbrM.33.3550   delta-12 fatty acid desaturase
Leishmania braziliensis LbrM.10.0010   fatty acid desaturase, putative
Leishmania braziliensis LbrM.10.1470   fatty acid desaturase, putative
Leishmania donovani LdBPK_333420.1   fatty acid desaturase, putative
Leishmania donovani LdBPK_101460.1   fatty acid desaturase, putative
Leishmania infantum LinJ.10.1460   fatty acid desaturase, putative
Leishmania infantum LinJ.33.3420   delta-12 fatty acid desaturase
Leishmania major LmjF.10.1320   fatty acid desaturase, putative
Leishmania major LmjF.10.0010   fatty acid desaturase, putative
Leishmania major LmjF.33.3270   delta-12 fatty acid desaturase
Leishmania mexicana LmxM.10.1320   fatty acid desaturase, putative
Leishmania mexicana LmxM.32.3270   fatty acid desaturase, putative
Loa Loa (eye worm) LOAG_02175   fatty acid desaturase
Oryza sativa 4343026   Os07g0416900
Oryza sativa 4343027   Os07g0417200
Oryza sativa 4330523   Os02g0716500
Trypanosoma brucei gambiense Tbg.972.2.1450   fatty acid desaturase, putative,oleate desaturase, putative
Trypanosoma brucei Tb927.2.3080   cytochrome b5-dependent oleate desaturase
Trypanosoma congolense TcIL3000_2_380   cytochrome b5-dependent oleate desaturase
Trypanosoma cruzi TcCLB.508737.70   cytochrome b5-dependent oleate desaturase
Trypanosoma cruzi TcCLB.429257.20   fatty acid desaturase, putative

Essentiality

Tb927.2.3080 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.2.3080 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.2.3080 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.2.3080 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.2.3080 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_W02A2.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_W02A2.1 Caenorhabditis elegans larval lethal wormbase
CELE_W02A2.1 Caenorhabditis elegans slow growth wormbase
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Reagent:
  • Target Type Source Notes
    Tb927.2.3080 cloned gene BRENDA A gene with this EC number or name or sequence has been cloned from Trypanosoma brucei ( 1 )

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.2.3080 (Trypanosoma brucei), cytochrome b5-dependent oleate desaturase
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